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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
02/03/2017 |
Actualizado : |
07/03/2017 |
Autor : |
GARCIA, J.P; GIANNITTI, F.; FINNIE, J.W.; MANAVIS, J. .; BEINGESSER, J.; ADAMS ,V.; ROOD, J.I.; UZAL, F.A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Comparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Veterinary Pathology, 2015, v.52.n.6. p.1250-1253, 2015. |
Idioma : |
Inglés |
Contenido : |
Abstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. MenosAbstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated wi... Presentar Todo |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
Asunto categoría : |
-- |
Marc : |
LEADER 02512naa a2200217 a 4500 001 1056750 005 2017-03-07 008 2015 bl uuuu u00u1 u #d 100 1 $aGARCIA, J.P 245 $aComparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives.$h[electronic resource] 260 $c2015 520 $aAbstract Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. 650 $aENFERMEDADES DE LOS ANIMALES 700 1 $aGIANNITTI, F. 700 1 $aFINNIE, J.W. 700 1 $aMANAVIS, J. . 700 1 $aBEINGESSER, J. 700 1 $aADAMS ,V. 700 1 $aROOD, J.I. 700 1 $aUZAL, F.A. 773 $tVeterinary Pathology, 2015$gv.52.n.6. p.1250-1253, 2015.
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
Fecha actual : |
18/10/2018 |
Actualizado : |
11/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
LOPEZ VALIENTE, S.; MARESCA, S.; RODRÍGUEZ, A.M.; PALLADINO, R.A.; LACAU-MENGIDO, I.M.; LONG, N.M.; QUINTANS, G. |
Afiliación : |
S. LÓPEZ VALIENTE, National Institute of Agricultural Technology (INTA). Experimental Station of Cuenca del Salado.; S. MARESCA, National Institute of Agricultural Technology (INTA). Experimental Station of Cuenca del Salado.; A.M. RODRÍGUEZ, National Institute of Agricultural Technology (INTA). Experimental Station of Cuenca del Salado.; R.A. PALLADINO, Facultad de Agronomía, Universidad de Buenos Aires. Facultad de Ciencias Agrarias, Universidad Nacional de Lomas de Zamora, Buenos Aires.; I.M. LACAU-MENGIDO, Laboratorio de Regulación Hipofisaria, Instituto de Biología y Medicina Experimental (CONICET).; N.M. LONG, Department of Animal and Veterinary Sciences. Clemson University.; GRACIELA QUINTANS ILARIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Effect of protein restriction of Angus cows during late gestation: subsequent reproductive performance and milk yield. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
The Professional Animal Scientist, 2018, 34(3): 261-268. |
DOI : |
10.15232/pas.2017-01701 |
Idioma : |
Inglés |
Contenido : |
Abstract:
The effect of level of CP fed during late gestation on reproductive performance and milk production was studied in multiparous cows. Sixty-eight pregnant Angus cows were used. At 121 d prepartum, cows were blocked by BW (409 ± 57 kg) and expected calving date, randomly assigned to a low-protein (LP = 6% CP) or high-protein diet (HP = 12% CP), and allocated to 12 pens per treatment.
After parturition, all cows were managed in a single group until weaning. Body weight and BCS were determined at the start of the experiment, at calving, and at weaning.
Nonesterified fatty acids, insulin, IGF-1, and glucose were determined every 24 d prepartum and nonesterified fatty acids and glucose every 38 d postpartum. Progesterone was quantified weekly to indicate luteal activity and estimate interval to first estrus. Milk production was measured until weaning. The HP cows had greater BW gain during the prepartum period (P < 0.01) and tended to gain more BCS (P = 0.06) than LP cows. The prepartum diet did not affect gestation length (P = 0.44) or interval from calving to the onset of luteal activity (P = 0.35).
Pregnancy rates, milk quality, and production were not influenced by dietary treatments. Cows in the HP treatment had greater prepartum serum urea concentrations than LP treatment (P < 0.05). In conclusion, protein level prepartum in multiparous beef cows affected the BW change at calving, without consequences on reproductive performance and milk quality and yield. |
Palabras claves : |
LATE GESTATION; MILK YIELD; MULTIPAROUS COW; POSTPARTUM REPRODUCTIVE PERFORMANCE; PROTEIN RESTRICTION; VACAS MULTÍPARAS. |
Thesagro : |
BOVINOS; VACAS. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
Marc : |
LEADER 02405naa a2200301 a 4500 001 1059651 005 2019-10-11 008 2018 bl uuuu u00u1 u #d 024 7 $a10.15232/pas.2017-01701$2DOI 100 1 $aLOPEZ VALIENTE, S. 245 $aEffect of protein restriction of Angus cows during late gestation$bsubsequent reproductive performance and milk yield.$h[electronic resource] 260 $c2018 520 $aAbstract: The effect of level of CP fed during late gestation on reproductive performance and milk production was studied in multiparous cows. Sixty-eight pregnant Angus cows were used. At 121 d prepartum, cows were blocked by BW (409 ± 57 kg) and expected calving date, randomly assigned to a low-protein (LP = 6% CP) or high-protein diet (HP = 12% CP), and allocated to 12 pens per treatment. After parturition, all cows were managed in a single group until weaning. Body weight and BCS were determined at the start of the experiment, at calving, and at weaning. Nonesterified fatty acids, insulin, IGF-1, and glucose were determined every 24 d prepartum and nonesterified fatty acids and glucose every 38 d postpartum. Progesterone was quantified weekly to indicate luteal activity and estimate interval to first estrus. Milk production was measured until weaning. The HP cows had greater BW gain during the prepartum period (P < 0.01) and tended to gain more BCS (P = 0.06) than LP cows. The prepartum diet did not affect gestation length (P = 0.44) or interval from calving to the onset of luteal activity (P = 0.35). Pregnancy rates, milk quality, and production were not influenced by dietary treatments. Cows in the HP treatment had greater prepartum serum urea concentrations than LP treatment (P < 0.05). In conclusion, protein level prepartum in multiparous beef cows affected the BW change at calving, without consequences on reproductive performance and milk quality and yield. 650 $aBOVINOS 650 $aVACAS 653 $aLATE GESTATION 653 $aMILK YIELD 653 $aMULTIPAROUS COW 653 $aPOSTPARTUM REPRODUCTIVE PERFORMANCE 653 $aPROTEIN RESTRICTION 653 $aVACAS MULTÍPARAS 700 1 $aMARESCA, S. 700 1 $aRODRÍGUEZ, A.M. 700 1 $aPALLADINO, R.A. 700 1 $aLACAU-MENGIDO, I.M. 700 1 $aLONG, N.M. 700 1 $aQUINTANS, G. 773 $tThe Professional Animal Scientist, 2018, 34(3): 261-268.
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